Cy3 wavelength excitation emission
Web20 rows · * Typical emission color seen through the eyepiece of a … WebThe Cy3 fluorophore is also the basis for the lipophilic neuronal and long-term DiI cell tracing reagents, which append alkyl tails (≥12 carbons) onto the core fluorophore. Invitrogen Alexa Fluor 555 dye is a Cy3 dye alternative that is generally more fluorescent and more photostable than Cy3 dye, making it well suited for applications such ...
Cy3 wavelength excitation emission
Did you know?
Web164 rows · Within groups, roughly in order of excitation wavelength (families excepted). Peak excitation and emission wavelengths often vary depending on the environment … WebApr 14, 2024 · Emission spectrum In the excited state, the electron loses some energy as heat. The remaining energy is released as a photon of light when the electron returns to the ground state. This causes the fluorochrome to emit light. The emitted light has less energy than the excitation light since some energy was lost as heat.
WebGeneral description. Atto 550 is a new label with high molecular absorption (120,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. Atto 550 can be used with similar excitation source and fluorescence filters as Cy3 ® and is characterized by a high photostability. find more information here. WebInvitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 dye or Invitrogen Alexa Fluor 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.
WebCy3 (Cyanine-3) is a fluorescent compound with an excitation peak at 555 nm and an emission peak at 569 nm. It can be excited using a 561 nm laser paired with a 585/20 …
Web400/30 excitation, 508/20 emission filter set (Figure 2B). Likewise, a much larger drop in signal (75%) is observed when the fluorescence of EGFP is determined with the 400/30 …
WebAlexa Fluor 647 dye. Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high ... selling iron condor on mcdonaldWebFirst Emission Wavelength. Blue (440 - 479nm) (3) Apply Blue (440 - 479nm) filter ; Fluorochrome. Acridine Orange + DNA (3) Apply Acridine Orange + DNA filter ; Alexa Fluor 350™ (3) Apply Alexa Fluor 350™ filter ; selling iron packWebWavelength (nanometers) Excitation Emission PE-Cy 5® Laser line (488) Tandem (PE-Cy5 ® ) Excitation and Emission Spectral Profiles Energy Level Diagrams Fluorochromes … selling irish babiesWebCy3 Filter Cube Sets comes with an excitation, emission, and dichroic filter for use with Cy3 fluorophores. Shop now with Edmund Optics! How may I help you? Engineers are … selling iron weightsWebFAM (Carboxyfluorescein) is a fluorescent compound with an excitation peak at 493 nm and an emission peak at 517 nm. It can be excited using a 488 nm laser paired with a 530/30 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta™. FAM (Carboxyfluorescein) is spectrally similar to Calcein Green, YOYO … selling is mcqWebDec 12, 2024 · To our knowledge, the Cy3–TQ3 pair has not been applied to structural studies of RNAs or proteins. Figure 1. FRET response of TQ3/Cy3-fl upon environmental change. ( A) Spectral overlap between the emission spectrum of Cy3 (red) with the excitation spectrum of TQ3 (blue). selling is both an art and scienceWeb400/30 excitation, 508/20 emission filter set (Figure 2B). Likewise, a much larger drop in signal (75%) is observed when the fluorescence of EGFP is determined with the 400/30 excitation, 508/20 emission filter set rather than its recommended filter set of 485/20 excitation, 530/25 emission filters (Figure 2A). A similar drop in signal would be ... selling is hard buying is harder