Flag antibody cst
WebApr 12, 2024 · We first developed a novel anti-FAP antibody using phage display technology. The binding specificity of the anti-FAP antibody was tested on 3T3-mFAP (Flag) and HT1080-huFAP. The results in Fig. 3a indicated that 8E3-scFv bound specifically to FAP-expressing cells but not to cells without FAP expression. WebThe family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3 (1). Neurotrophin signaling through these receptors regulates a number of physiological processes, such …
Flag antibody cst
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Web2 days ago · Primary antibodies used in this study were as follows: anti-MYC (9B11, Cell Signaling, mouse monoclonal), anti-Flag (F1804, Sigma-Aldrich, mouse monoclonal); secondary antibodies used were Alexa ... WebFocal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397 ...
WebImmunohistochemical analysis of paraffin-embedded mouse small intestine using Tom20 (D8T4N) Rabbit mAb. Confocal immunofluorescent analysis of HeLa (high expression, left) and Saos-2 (low expression, right) cells, using Tom20 (D8T4N) Rabbit mAb (green). Actin filaments were labeled with DyLight ™ 554 Phalloidin #13054 (red). WebWB. Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa cells using MLKL (D2I6N) Rabbit mAb #14993. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the …
WebThis product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, … WebMonoclonal Antibody for studying Flag-Tag. Cited in 2 publications. Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, DYKDDDDK Tag (D6W5B) Rabbit Monoclonal Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Sepharose® Bead Conjugate) (CST #70569) is ready to ship.
WebMonoclonal Antibody for studying Flag-Tag. Cited in 855 publications. Validated for WB, IP, IHC, IF, F, ChIP. Highly specific and rigorously …
Web6th Sep, 2016. Olga Mikhaylichenko. King's College London. Hi Jinhu, yes, you can use the beads coupled to the antibody directly for your ChIP experiment. Incubate your lysates with the beads ... gaming pc prices in pakistanWebSpecificity / Sensitivity. K48-linkage Specific Polyubiquitin Antibody detects polyubiquitin chains formed by Lys48 residue linkage. Antibody demonstrates slight cross-reactivity with linear polyubiquitin chain. No cross-reactivity observed with monoubiquitin or polyubiquitin chains formed by specific linkage to different lysine residues. gaming pc ram speedWebApr 14, 2024 · Secondary antibodies (Mouse IgG HRP Linked Whole Ab, NA931, Cytiva, Tokyo, Japan or Anti-rabbit IgG, HRP-linked Antibody #7074, Cell Signaling), were used at a dilution of 1:2000 for 1 h at room ... gaming pc project carsWebJan 11, 2013 · Antibodies against XBP1 (M-186), CD31, and GAPDH were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA), whereas antibody against FLAG was from Abcam, and anti-LC3 (M186-3) was from MBL. Antibodies against IRE1α, pIRE1α, and BECLIN-1 (Autophagy Kit-4445) were purchased from Cell Signaling. Endostatin … gaming pc refurbished canadaWebMonoclonal Antibody for studying IFN-beta1 mouse. Cited in 4 publications. Validated for Western Blotting, Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IFN-β1 (D2J1D) Rabbit Monoclonal Antibody (CST #97450) is … gaming pc prozessor testWebMar 22, 2024 · The protein levels of EGFR, FLAG and β-actin in whole cell lysate (WCL) and Biotin fractions were detected with immunoblotting. The data showed two independent experiments (1st and 2nd). b HEK293T cells were transfected with FLAG-DRAM1 plasmid for 24 h, the cells were fixed and stained with FLAG antibody (red). Representative … black holes in tomatoesWebI'm using the anti-FLAG AB from Cell Signaling (1:1000 overnight), which detects the same epitope as Sigma's AB. ... Another option would be trying to IP with the flag antibody and blot with the ... gaming pc programs need