2024 Gm-130 antibody-directed staining

Gm-130 antibody-directed staining

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August undefined, 2024

WebIndependent Antibodies: Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human cerebral cortex, colon, lymph node and testis using … WebIn biochemistry, immunostaining is any use of an antibody -based method to detect a specific protein in a sample. The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue …

GM130 Antibody Cell Signaling Technology

WebFixation / permeabilization. Optimize protocol for best results with both antibodies. Separate testing may be required. Rinse. Dilute each antibody to its recommended dilution in antibody dilution buffer; incubate overnight at 4°C. Rinse. Perform any counterstaining steps using complementary reagents, mount in anti-fade reagent. The pros and ... WebII. Primary Antibody Incubation. Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product webpage) in 10 ml … how a brake light switch works

Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] (ab195303)

WebThis highly specific GM130 antibody is suitable for use in WB, IHC-P and Direct ELISA with human, mouse and rat samples and is guaranteed to work as stated on the product data sheet. RQ4570 ... Immunofluorescent staining of FFPE human U-2 OS cells with GM130 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate ... WebAntibodies that detect GM130 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Flow Cytometry. These antibodies target GM130 in Human, Rat, Mouse and Canine … WebGOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and … how a brake fluid tester operates

Anti-GM130 Antibodies Invitrogen - Thermo Fisher …

CDC - Diagnosis: Direct Fluorescent Antibody Test

WebDec 5, 2024 · GM130, a cis-Golgi matrix protein, interacts specifically with p115 and provides a membrane docking site. Both GM130 and p115 are involved in vesicle tethering to Golgi membranes. The amino-terminus of GM130 binds to p115, whereas the carboxy-terminus binds to Golgi membranes. Both Giantin and GM130 compete for binding to p115. WebProduct name Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker See all GM130 primary antibodies Description Alexa Fluor® 647 Rabbit monoclonal [EP892Y] to GM130 - cis-Golgi Marker Host species Rabbit Conjugation Alexa Fluor® 647. Ex: 652nm, Em: 668nm Tested applications Suitable for: ICC/IF, Flow Cyt (Intra) more details how many have bought rocket leagueWebGM130 Polyclonal Antibody detects GM130 protein at Golgi apparatus and endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GM130 protein stained by GM130 Polyclonal Antibody ( Product # PA5-85643) diluted at 1:200. Blue: Hoechst 33342 staining. how a brain looks like

"WebWhen antibodies bind to or block these targets they disrupt functioning, resulting in neurologic and psychiatric symptoms. 6. The autoantibodies may also trigger inflammation (encephalitis) in the basal ganglia. This … " - Gm-130 antibody-directed staining

Gm-130 antibody-directed staining

GM130 gain-of-function induces cell pathology in a model of …

Web3.317.3.1.1 Fluorophore-tagged antibodies. Immunostaining is a general term in biochemistry that applies to any use of an antibody-based method to detect a specific … WebImmunofluorescent staining of human cell lines. HeLa cells (ATCC CCL-2) were cultured, fixed, permeabilized with Triton™ X-100, stained with Alexa Fluor® 488 Mouse anti-GM130 (pseudo colored green), and counter stained with Hoechst 33342 (pseudo colored blue) according to the Recommended Assay Procedure.

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WebFeb 5, 2024 · Immunohistochemistry (IHC) is based upon the specific detection of an antigen, using an antibody to recognize it and then staining the resulting complex. This is why it is also called... WebWhole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with …

WebNational Center for Biotechnology Information WebAnti-GM130 (green) antibodies identified GM130-expressing and GM130-depleted cells. Nuclei were counterstained in blue. When compared with non-transfected cells visible on the same field, merged apotome views showed higher giantin staining in cells positive for FLAG tag (arrowheads), and lower giantin staining in cells negative for GM130 ...

WebIntroduction. Cold-antibody autoimmune hemolytic anemias (cAIHAs) are mediated by autoantibodies characterized by a temperature optimum of the antigen-antibody (AgAb) … WebRabbit Polyclonal GM130 antibody. Validated in WB, ICC/IF, IP. Tested in Human. Cited in 3 reference(s). United States (US) Country / Location Selection Product Research ... Blue: Hoechst 33342 staining. GTX130351 IP Image Immunoprecipitation of GM130 protein from HeLa whole cell extracts using 5 μg of GM130 antibody (GTX130351). ...

WebAnti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370) Protein Recombinant Human GM130 protein (ab131818) Primary Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966) View more associated products Overview Product name Alexa Fluor® 488 Anti-GM130 antibody [EP892Y]

WebGM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry. … how a brake booster worksWebIntracellular flow cytometry applications for GM-CSF Antibody, anti-human APC PE Conjugate: APC Recommended dilution: 1:10 Remark: The antibody is suited for staining of formaldehyde-fixed cells. Tested: QC tested Products used: 130-099-625, 130-096-078 Protocols: Intracellular flow cytometry staining protocol (Inside Stain Kit 1:10, protocol A) how a brain worksWebAnti-PARP antibody was used as a control for nuclear proteins, and anti-Tubulin antibody was used as a control of cytoplasmic proteins. B, … how a brake switch works how many have climbed everest without oxygenWebApr 6, 2024 · Immunogold staining was employed to detect the PI4P on sEVs. The results showed that the PI4P antibody labels were observed on the sEVs. The GFP labels of the GFP-tagged PI4P sensor were also observed on sEVs, while the secondary antibody negative control was not labelled (Figure 1g). The antibody cannot penetrate the cell … how abrasion causes weatheringWebMay 15, 2024 · Antibodies used at too high of a concentration can result in off-target staining.32–35 While determination of signal to noise ‘by eye’ is often used, to be … howa bravo 6.5 creedmoorWebMay 12, 2024 · Antigen detection by dFA. The rabies antibody used for the dFA test is primarily directed against the nucleoprotein (antigen) of the virus (see The Virus section on viral structure). Rabies virus replicates in the … how a brake chamber works