WebApr 7, 2024 · All RNA extracts were then diluted to produce extracts that were 2.0 ng μl −1 prior to being used in quantitative reverse-transcription PCR (qRT-PCR) assays targeting the Wolbachia 16S rRNA gene . A synthetic oligonucleotide standard (Integrated DNA Technologies) was designed to calculate 16S rRNA gene copies per µl using a 10-fold … Aphids of each population were collected from a mature gall. Fresh aphid galls were collected from host plants manually and dissected in the lab. Aphids were transferred to an Eppendorf tube from a gall, placed in 100% ethanol and then stored at − 20 °C until DNA extraction. Galls collected from each location within ten … See more Ten to twenty aphid individuals of a population were selected for DNA extraction. Genomic DNA was extracted using Dzup Genomic DNA Isolation Reagent (Sangon … See more Nine genes 16S rRNA, gltA, groEL, wsp, gatB, fbpA, coxA, hcpA and ftsZ of Wolbachia were amplified and sequenced from the populations harboring Wolbachia. PCR products were sequenced from both directions in an ABI … See more DNA samples of 117 natural galling aphid populations were screened for the presence of Wolbachia strains. Detection was based on the amplification of the 16S rRNA gene fragment … See more Twelve gene sequences of 16S rRNA, gltB, fbpA, coxA, hcpA and ftsZgenerated in this study have been deposited in the GenBank database under accession numbers MT554837 … See more
Identification and Biological Role of the Endosymbionts Wolbachia …
WebOct 5, 2024 · How do Wolbachia affect host reproduction? Cytoplasmic incompatibility caused by males is associated with interference in embryonic nuclear division. A … WebAug 1, 2000 · Long PCR, which uses two enzymes (Taq and Pwo), consistently amplified Wolbachia DNA and a sensitivity analysis indicated that Long PCR was approximately … magnolia ar hospital
Distribution of the Reproduction-modifying Bacteria, Wolbachia, …
WebApr 14, 2024 · If these tested positive, the specimens of the pools were individually analyzed. For molecular detection of Wolbachia strains, we amplified a fragment of the … WebApr 12, 2024 · The main cause of ADO is the failure of primers to anneal and amplify the target location either by sequence alteration in the target sequence or by suboptimal PCR conditions. Let’s understand each scenario, one after another. Genetic alteration: Sequence variation or mutation in any of the alleles of the target sequence cause allelic dropout. WebAug 21, 2024 · Some bacteria such as Wolbachia are currently being considered for the control of Dengue, ... Wolbachia 16S rRNA was also amplified and sequenced from one adult of Ae. ... Wolbachia PCR. magnolia ar homes