Webb15 mars 2024 · Cells were treated with 20 ng ml –1 KaryoMAX colcemid solution (Thermo Fisher Scientific, 15212012) for 2 h at 37 °C in a humidified incubator. Cells … WebbGibco® KaryoMAX® Colcemid™ Solution in HBSS is a 10 µg/mL N-desacetyl-N-methylocolchicine (Colcemid™) solution made up in Hanks’ Balanced Salt Solution (HBSS). Colcemid™ prevents spindle …
15212012 - KaryoMAX Colcemid Solution in PBS at Rs 1874/unit ...
Webb11 apr. 2024 · For both passages, 70–80% confluence was reached. Then, the culture medium was changed and supplemented with 10 μg/mL colcemid solution (KaryoMAX ® Colcemid™ Solution, Gibco ®, Waltham, MA, USA). After 4 h, the eSM-MSCs were collected and resuspended in 8 mL of 0.075 M KCl solution, followed by incubation … WebbGibco® KaryoMAX® Colcemid™ Solution in HBSS is a 10 µg/mL N-desacetyl-N-methylocolchicine (Colcemid™) solution made up in Hanks’ Balanced Salt Solution (HBSS). Colcemid™ prevents spindle formation during mitosis, arresting cells in metaphase so that the chromosomes can be separated for cytogenetic studies and in … the pink lodge
Gibco™ KaryoMAX™ Colcemid™ Solution in HBSS
WebbAfter an optimum time of the cells being cultured (46 h for a newborn and 68 h for an adult), a mitotic inhibitor, KARYOMAX COLCEMID® Solution (Cat. No. 15210 or 15212), is added to the lymphocyte culture for 20 min. Webb11 apr. 2024 · Megnevezések Vizsgálat /év Vizsgálat X-chromatin meghatározás 277 Y-chromatin meghatározás 125 Chromosoma analízis rövid idejű sejttenyésztéssel 115 … Webb26 aug. 2024 · KaryoMAX™ Colcemid™ Solution in PBS (Gibco). 10. Hypotonic buffer (KCl 0.075 M). 11. Fixative (3 volumes of methanol: 1 volume of acetic acid, freshly prepared). 12. 15 ml centrifuge tubes. 13. 1.5 ml microcentrifuge tubes. 14. Aluminum foil. 15. Tabletop centrifuge (Beckman Allegra X-12 Tabletop Centrifuge) and … the pink lotus breast center