Phellsgate 4
WebCN106755018A CN201510836230.6A CN201510836230A CN106755018A CN 106755018 A CN106755018 A CN 106755018A CN 201510836230 A CN201510836230 A CN 201510836230A CN 106755018 A CN106755018 A CN 106755018A Authority CN China Prior art keywords cotton gene ghppc genes medium Prior art date 2015-11-25 Legal … WebJan 1, 2005 · The pHELLSGATE vectors contain convenient restriction sites that can be used to check the presence of inserted sequences and diagnose the orientation of the intron …
Phellsgate 4
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WebA series of HELLSGATE vectors hve been generated ( Fig. 4 ). Constructs in pHELLSGATE4 are generated by a single recombination with an attB-flanked PCR product; however, more effective silencing is observed with constructs in pHELLSGATE 8 or 12. WebIn this paper we describe two improved pHellsgate vectors that facilitate rapid generation of hpRNA-encoding constructs. pHellsgate 4 allows the production of an hpRNA construct in …
WebPhellsgate 2 Vector Thermo Fisher Bioz Bioz Stars score, Techniques, Protocol Conditions and more for Phellsgate 2 Vector, supplied by Thermo Fisher. Data for Phellsgate 2 … WebZestimate® Home Value: $677,100. 1104 Fieldgate Ave, La Puente, CA is a single family home that contains 1,056 sq ft and was built in 1956. It contains 3 bedrooms and 2 …
WebAug 1, 2003 · The pHELLSGATE vectors contain convenient restriction sites that can be used to check for the presence of inserted sequences and diagnose the orientation of the … WebpHELLSGATE 4 Improved high-throughput binary Gateway® donor vector for silencing genes in plants using intron-containing hairpin RNA. Open in SnapGene Try SnapGene for Free …
WebThe 311bp PCR product was cloned into the pHellsgate4 plasmid through the Gateway BP recombination reaction to obtain the GhGRAM31-RNAi vector (Helliwell et al., 2002). This vector was then...
WebOct 1, 2002 · In this paper we describe two improved pHellsgate vectors that facilitate rapid generation of hpRNA-encoding constructs. pHellsgate 4 allows the production of an … diamondback sidekick 22 calWebThe system utilizes a modified pHELLSGATE vector, under the control of the pOp6 promoter, and the synthetic transcription factor, LhGR. We demonstrate that the production of RNAi … circle saw millsWebJan 16, 2014 · pGFP plant was transformed in our Uzbekistan laboratory with green fluorescent protein (GFP) construct (pBINmGFP5-ER ) used in cotton transformation 12; somatically regenerated PCR-negative... circle saw cutting jigWebMay 31, 2012 · Here, we describe a novel restriction-ligation approach that provides a simple but efficient construction of intron-containing hpRNA (ihpRNA) vectors. The system takes advantage of the type IIs restriction enzyme BsaI and our new plant RNAi vector pRNAi-GG based on the Golden Gate (GG) cloning. circle sawn white oak flooringWebSep 9, 2024 · Fig. 4 Whisker box plot analysis of PAL1 expression levels in ASM- and water-treated samples (Kaleso and Kalawe combined) normalized to respective 0 dat, at different time points. Significant difference in PAL1 gene expression was observed between the ASM- and water-treated plants (p < 0.001), 0.3 day = 8 hat Full size image circle saw repairWebAug 1, 2015 · C12N15/825 Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic... diamondback sidekick 22 priceWebApr 15, 2004 · A Gateway vector, pHELLSGATE, which they developed for high-throughput RNA silencing, contains the 35S promoter. More recently the same group developed various derivatives of pHELLSGATE for efficient construction and gene suppression ( Helliwell and Waterhouse 2003 ). diamondback sidekick 22 caliber revolver