Phospholipidosis detection reagent
WebApr 1, 2015 · Phospholipidosis (PLD) is a disorder of excessive storage of phospholipids (PLs) in the lysosomes of cells and tissues. Several mechanisms have been described which may lead to the accumulation of PLs in lysosomes and can be due to genetic disorders (e.g. Niemann-Pick disease) or triggered by xenobiotics or drugs, referred to as drug-induced … WebMar 1, 2024 · The EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large …
Phospholipidosis detection reagent
Did you know?
WebDec 7, 2024 · Annexin V-FITC/PI apoptosis detection kit was acquired from BD (Franklin Lakes, NJ, USA). Then, Hoechst 33342, eBioscience™ JC-1 Mitochondrial Membrane Potential Dye, Cell ROX Deep Red, and HCS LipidTOX™ Green Phospholipidosis Detection Reagent were obtained from Thermo Fisher Scientific (Waltham, MA, USA). WebDec 17, 2016 · Drug-induced phospholipidosis caused by combinations of common drugs in vitro.药物引起phospholipidosis引起的常见药物在体外 ... combination limitmea- surement mistakes. cellswere further24 concomitantviability detection, 11 PrestoBlueCellViability Reagent (PrestoBlue) (Invitrogen, Carlsbad, US) were added eachwellafter 22 hto ...
WebInvitrogen™ HCS LipidTOX™ Green Phospholipidosis Detection Reagent (1000X), for cellular imaging. Easily detected by fluorescence microscopy. Manufacturer: Invitrogen™ … WebThe HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect excitation/emission maxima ∼595/615 nm.
WebMar 1, 2024 · LipidTOX Green Phospholipidosis Detection Reagent (LipidTOX, Invitrogen, Carlsbad, CA, USA) was applied to confirm induction of phospholipidosis by selected … WebSeveral methods for the detection of phospholipids have been reported (29-37). They are summarized in Table 1. In-vitro screening assays using the fluorescently tagged lipid NBD-PE, the fluorescent probe Nile red as well as the phosphatidylcholine-conjugated dye NBD-PC were previously used to determine intracellular phospholipids (29, 30, 34).
WebThe LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid stains can also be used to monitor the formation and ...
WebThe EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical … ingles grocery store raleighWebInitial experiments focused on the response of human mesothelioma cells to agents known to cause drug-induced phospholipidosis. When H-meso cells were treated with various concentrations of either chloroquine or verapamil a dose dependent increase in Lyso-ID Red signal is observed (Figure 5). mitsubishi mini split systems wifiWebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and differentiation of adipocytes, a process called adipogenesis. Cell Analysis, Cellular Imaging, High-Content Screening (HCS) Order Info Shipping Condition: Room temperature Specifications ingles grocery store powdersville scWebThe EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical library. This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. ingles grocery store near me ashevilleWebJul 16, 2015 · For phospholipid staining, the cells were treated with 400 μM palmitate containing 1X LipidTOX Red phospholipidosis detection reagent. For Immunostaining with an anti-calnexin antibody, the cells were fixed in 4 % paraformaldehyde in PBS. The fixed cells were rinsed with PBS and incubated in blocking solution (5 % goat serum, and 0.001 … ingles grocery store north coveWebPhospholipidosis is an excessive intracellular accumulation of phospholipids and drug, which is normally reversible after discontinuation of drug treatment. Currently, it is … mitsubishi mini split systems specificationsWebLipidTox Green neutral lipid stain and LipidTox Red phospholipidosis detection reagent (Kit from Molecular Probes) ... ORIGINAL ARTICLE 232 E-LDL and Ox-LDL Differ in the Lipid Storage. phospholipidosis detection depending on the emission spectra of the dye (Table 1) (30,37–39). Lipid-loaded macrophage foam cells play a major role in mitsubishi mini split systems registration