2024 Pma and hl-60

Pma and hl-60

Author: yaie

August undefined, 2024

Web摘要：目的: 研究β2-糖蛋白Ⅰ(β2-gpⅠ)对THP-1来源的巨噬细胞吞噬凋亡HL-60细胞的影响,并探讨其作用机制. 方法: 体外培养人急性髓系白血病细胞株HL-60,用阿糖胞苷(Ara-C)诱导其凋亡并用MTT法和AnnexinV-PI联合染色法检测细胞生长抑制率和凋亡情况;体外培养人急性单核细胞白血病细胞株THP-1,用佛波酯(PMA ... WebHL-60 cells were seeded in 96-well tissue culture plates (2 3 104 cells/well, 100 ml) in RPMI 1640 medium supplemented with 10% FCS and kanamycin (50 mg/ml). The cells were incubated with indicated concentrations of PMA or Me 2 SO for 0, 12, 24, or 48 h. Because PMA was dissolved in 0.1% (v/v) Me 2 SO, this solution served as a control for PMA.

Differentiation of HL‑60 cells in serum‑free hematopoietic cell media e…

WebThe protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in 1640 RPMI +... Webrs.yiigle.com body works black friday

The influence of agents differentiating HL‐60 cells toward …

WebJan 6, 2010 · When HL-60 cells were induced with PMA to differentiate into macrophage-like cells, we found that actin redistribution occurred. Western blot analysis using whole-cell lysates revealed that total β-actin content increased by 1.48 ± 0.16-fold HL-60 cells treated with PMA for 24 h, and the content of β-actin remained stable after 24 h of ... WebMA-2250 INCOME. MA-2260 FINANCIAL ELIGIBILITY REGULATIONS-PLA. MA-2311 LIS PROCESSING AND MAINTENANCE. MA-2360 MEDICAID DEDUCTIBLE. MA-2525 … WebJan 13, 2010 · The promyelocytic leukaemia cell line HL-60 differentiates towards a monocytic phenotype in response to VD 3 or a macrophage-like cell in response to phorbol esters, such as PMA . PMA treatment, which activates protein kinase C (PKC), also induces a greater degree of differentiation in THP-1 cells as reflected by increased adherence and ... glitter acrylic powder for nails

Culturing HL-60 Cells on RPMI 1640 : r/labrats - Reddit

The Identification of Markers of Macrophage Differentiation in PMA …

WebDec 9, 1997 · 1.. IntroductionThe human leukemia HL-60 cell line is a useful model to investigate the mechanism of cell differentiation. HL-60 cells are induced to differentiate into macrophages by PMA 2, 8.This differentiation is accompanied by an overall decrease in the phosphotyrosine level in the cells [3].It has been shown that the expression of PTP1C, … WebFeb 19, 2024 · NET-formation of PMA-stimulated primary neutrophils and 3 days DMSO-differentiated HL-60 cells. a NET formation by HL-60 cells differentiated with DMSO for 3 … body works black friday saleWebMay 19, 2000 · Morphologic differentiation of HL-60 cells is associated with appearance of RPTPbeta and induction of Helicobacter pylori VacA sensitivity. Phorbol 12-myristate 13 … glitter adhesive vinyl wholesale

"WebJun 17, 2002 · The phosphorylation of p85 α derived from PMA-stimulated HL-60 cells was shown in the time dependent manner. However, p70 S6 kinase inhibitor, rapamycin, did not inhibit PMA-induced monocytic differentiation. During PMA-induced monocytic differentiation, LY294002 inhibited c-jun protein expression and decrease of c-myc protein … " - Pma and hl-60

Pma and hl-60

Fibronectin-mediated cell adhesion is required for induction

WebJordan Priestley Mrs Priestley ICT (@mrspriestleyict) on Instagram: "Come and SEE my exhibitor set up for the PMA Seminar 燐 Lots to showcase & share for teachers..." Jordan Priestley Mrs Priestley ICT on Instagram: "Come and SEE my exhibitor set up for the PMA Seminar 🧮 Lots to showcase & share for teachers to use in their classroom! WebPMA is a direct activator of PKC. Treatment of normal human neutrophils6,7) and dimethylsulfoxide-diﬀerentiated HL-60 cells8) with PMA results in rapid generation of ROSs and subsequent cell death accom-panied by chromatin condensation and PS external-ization. This externalization is blocked in neutrophils from CGD patients.

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WebThe HL-60 cell line is a promyelocytic cell line derived from human leukemia (Collins, Gallo, & Gallagher, 1977 ). The cells are premyelocytes, but can be induced in vitro to … WebMar 21, 2024 · cd6 与cd3 或pma 共同剌激成熟t 淋巴细胞，参与自身免疫病和移植物抗宿主病的发生。 CD6 抗体能识别在80% 的成熟T 淋巳细胞和B 淋巴细胞亚型上表达的分子量为100 kD 的I型转移膜糖蛋白，还可用于白血病和淋巴瘤免疫分型研究及CD6+B 淋巴细胞功能的研究 …

WebMEK activation in HL-60 cells exposed to PMA or RA. Cells were treated with either PMA (a-c) or RA (d-f) and whole cell lysates were harvested at varying time-points. Protein aliquots (40... WebMar 13, 2024 · HL-60 is a promyelocytic cell line that differentiates into neutrophils in the presence of dimethyl sulfoxide (DMSO) and has been used extensively as a model to study neutrophil function, including superoxide anion production ( Cowen et al. 1991 ). Currently, the effects of Echinacea on superoxide anion production by HL-60 cells are unknown.

WebSep 16, 2011 · Effect of the aqueous extracts of Abelmoschus esculentus, Hibiscus acetosella, Manihot esculenta and Pteridium aquilinum on the ROS production by PMA activated HL-60 monocytes. Monocytes (10 6 /mL) were incubated for 45 min with 41 µM non-fluorescent DCFH-DA probe in the dark, centrifuged (300× g ) for 10 min at 37 °C and … WebNext to THP-1 and U937 cells, ML-2, HL-60 and Mono Mac 6 cells are used in biomedical research. U937 cells are the most frequently used. The basic difference ... 100 ng/ml PMA (162 nM), followed by washing twice with culture media without PMA and a resting period of 24 h (Chanput et al. 2014 ), resulting in macrophages ...

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WebMay 15, 2024 · We investigated the mechanisms and the role of autophagy in the differentiation of HL-60 human acute myeloid leukemia cells induced by protein kinase C … bodyworks bliss provoWebNov 6, 2015 · The monocyte/macrophage differentiation of THP-1 and HL-60 was induced with PMA (Sigma-Aldrich) at final concentration of 10 nM. The monocyte/macrophage differentiation culture of CD34 + HSPCs was ... body works boonsboroWebDual effects of tetrandrine on cytosolic calcium in human leukaemic HL-60 cells: intracellular calcium release and calcium entry blockade. ... 10 nM phorbol myristate acetate (PMA, a protein kinase C activator) or 100 nM staurosporine (a protein kinase C inhibitor) had no effect on 100 microM TET-induced intracellular Ca2+ release. 3. After ... glitter adhesive foamWeb• Antibody blocking of PMA-stimulated HL-60 cells infected with Myxoma Virus • Test for evidence of increased expression of cell-surface markers on HL-60 cells after PMA-stimulation. glitter adjectiveWebDec 22, 1998 · Human myeloid leukemia cell line HL-60 was originally obtained from R. C. Gallo (National Cancer Institute, Bethesda, MD). The differentiation-resistant cell variant HL-525 was obtained by subculturing HL-60 cells 102 times in the presence of increasing concentrations (up to 3 μM) of PMA at 5- to 8-day intervals (12). glitter adhesive vinyl hobby lobbyWebDec 22, 1998 · Macrophage maturation in HL-60 cells is initiated upon activation of protein kinase C (PKC), which acts as both a specific receptor and a functional signal … glitter acrylic sheetsWebMay 1, 1997 · PMA-stimulated HL-60 cells were prepared by the protocol described previously. Washed cells were resuspended in HBS containing 12.5 mmol/L CaCl 2 at a final concentration of 10 6 cells per mL and warmed to 37°C. The cells then received either 10 μmol/L ionomycin or vehicle (0.1% DMSO). glitter adhesive for eyes