Tnt nitroreductase
WebbIt is illustrated that pnrB may perform a crucial role in the TNT degradation pathway of the HK-6 strain by measuring absorbance changes at 340 nm due to NADH oxidation. Pseudomonas sp. HK-6 is able to utilize 2,4,6-trinitrotoluene (TNT) as a sole nitrogen source. The pnrB gene of the HK-6 strain was cloned using degenerate primers … Webb1 mars 2000 · Many enteric bacteria express a type I oxygen-insensitive nitroreductase, which reduces nitro groups on many different nitroaromatic compounds under aerobic conditions. Enzymatic reduction of nitramines was also documented in enteric bacteria under anaerobic conditions. This study indicates that nitramine reduction in enteric …
Tnt nitroreductase
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WebbThe substrate specificity test revealed that the nitroreductase exhibited the highest enzyme activity for the TNT substrate of the nitroaromatic compounds tested in this …
Webb1 aug. 2005 · In this study we have identified the nitroreductase-family enzymes PnrA and PnrB from the TNT-degrading strain Pseudomonas putida. The enzyme encoded by the pnrA gene was expressed in Escherichia coli, purified to homogeneity and shown to be a flavoprotein that used 2 mol of NADPH to reduce 1 mol of 2,4,6-trinitrotoluene (TNT) to 4 … Webb1 apr. 2024 · TNT is toxic to aquatic flora and other organisms and animals, including reptiles. TNT is classified as mutagenic, poisonous, and carcinogenic. Even at a very low concentration of 50 mg/L, TNT is lethal for microorganisms including Gram-positive bacteria and yeasts [ 7].
Webb1 nov. 2005 · Three NADP (H)dependent nitroreductases can transform 2,4,6-trinitrotoluene (TNT) by two reduction pathways were detected in Klebsiella sp. C1 by (Kim and Song 2005). Nitroaromatic compounds are... Webb19 mars 2008 · The nitroreductase NR from Enterobacter cloacae is a homodimer, although it is also active as a 27 kDa monomer, and shows apparent K m values for TNT …
Webb1 juni 2001 · TNT was rapidly degraded by an enzyme obtained from a Pseudomonas aeruginosa strain isolated from TNT-contaminated soil. The nitroreductase enzyme was …
WebbTNT (2,4,6-trinitrotoluene) is one of the most widely used military and civil explosives, which has a huge negative impact on the environmental ecosystems [Citation 1]. TNT, … imahorn andrea wilWebb1 dec. 2001 · Nitroreductase catalyzes the reduction of TNT to hydroxyaminodinitrotoluene (HADNT), which is subsequently reduced to aminodinitrotoluene derivatives (ADNTs). Transgenic plants expressing nitroreductase show a striking increase in ability to tolerate, take up, and detoxify TNT. im a hoot shirtWebb1 maj 2012 · Oxygen-insensitive nitroreductases are NAD (P)H dependent enzymes, usually a dimeric protein with FMN as a prosthetic group, and catalyze the reduction of nitro groups in a series of two-electron transfer processes to produce the corresponding nitroso, hydroxylamino, and amino derivatives [3]. list of gift items for employeesWebbof the total nitroreductase activity within the three peaks is approximately equal in both E. coli and S. typhimurium, the major peak isolated during the purification of the E. cloacae nitroreductase represents 90% of the total activity recovered. Thus, it appears that TNT induces the expression of a specific nitroreductase. im a hoot meaningWebb2,4,6-trinitrotoluene (TNT) and the involvement of nitroreductase (NR) enzyme in TNT degradation in vetiver has been documented in our earlier studies. The present study was designed to optimize the parameters influencing NR enzyme-mediated TNT transformation in vetiver. The current study is the first report of detailed kinetic exami- list of gift ideas for womenWebbNitroreductase I has a molecular mass of 27 kDa as determined by SDS-PAGE, and exhibits a broad pH optimum between 5.5 and 6.5, with a temperature optimum of 30 … im a horrible friens for doing that i knowWebbAbstract: Nitroreductase (NTR) is an enzyme that specifically reduces nitroaromatic activity. Some nitroaromatic explosives are exploited by terrorists. Among these highly … im a hot boy ho